ISOLATION AND IDENTIFICATION OF AFLATOXIN B1 PRODUCING FUNGI FROM STORED WHEAT IN SOME SILOS OF BAGHDAD

The aim of this study was isolation and identification fungi producing aflatoxin B1 from wheat which stored for four months in some silos Baghdad , including Al Taji Silo, Khan Bani Saad Silo Dhari Silo. Those Silos were included the agricultural plan marketing season 2020. Different types obtained. They initially identified according to cultural morphological characteristics as Alternaria spp, Aspergillus flavus niger. ability fungal isolates produced tested using ammonia vapor. All showed their produce aflatoxin, unlike other negative result. Aflatoxin by estimated (HPLC) technique, results that highest concentration (978.5 µg / ml) obtained an isolate Al-Taji This genetically genetic analysis 5.8S rRNA gene. Gene sequencing identity local 99% compassion strain USMG09 recorded gene bank ID: MK992254.1 .